首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人大肠癌专一抗原2(CCSA-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CCSA-2含量。试剂盒检测范围为3.33 ng/mL-80 ng/mL，适用于大肠癌发病机制研究、抗癌药物体外筛选、治疗效果评估及疾病动物模型中CCSA - 2动态变化分析。适用于实验室条件下探索CCSA - 2在大肠癌微环境调控、肿瘤侵袭性评价等领域的生物学功能。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：CCSA-2
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.33 ng/mL-80 ng/mL
灵敏度：1.33 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CCSA-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-100 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 86-101 | |||||
回收率：
| The recovery of human CCSA-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-102 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.0151.9701.9931.946401.3441.3581.3511.304200.7990.8010.8000.7536.670.3410.3590.3500.3033.330.2010.2150.2080.16100.0470.0470.047 | ||||||
货期：3-5 working days
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