首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人不对称二甲基精氨酸(ADMA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ADMA含量。ADMA即不对称二甲基精氨酸，是内源性一氧化氮合酶（NOS）的抑制剂。它可导致血管内皮功能障碍，在心血管疾病、肾脏疾病等多种疾病发生发展中起重要作用。目前研究关注其产生、代谢机制及降低其水平以改善疾病预后的策略。试剂盒检测范围为7.8 ng/mL-500 ng/mL，本产品适用于心血管疾病模型构建、代谢综合征机制研究、肾脏功能调控实验等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ADMA
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 94-105 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 89-97 | |||||
回收率：
| The recovery of human ADMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 87 | 82-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.7632.6122.6882.5522502.2492.1492.1992.0631251.4671.4281.4481.31262.50.8830.8230.8530.71731.20.4970.5050.5010.36515.60.3570.3860.3720.2367.80.2410.2290.2350.09900.1390.1320.136 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ADMA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled ADMA antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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