首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人补体片断4b(C4b)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的C4b含量。C4b是补体系统经典途径和凝集素途径激活过程产生的重要片段。补体系统在免疫防御等方面发挥关键作用。研究显示，C4b可参与调理吞噬、免疫黏附等过程，其异常活动可能与多种免疫相关疾病发生发展有关，是免疫学研究重要靶点。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于科研领域中对补体系统功能的探索，例如在炎症模型、免疫调节实验或疾病机制研究中定量分析血清/血浆样本的C4b表达动态，为揭示补体介导的生理病理过程提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Basic complement C4 ELISA Kit; C2B5 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4b ELISA Kit; C4B1 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; CO4B_HUMAN ELISA Kit; Complement C4 gamma chain ELISA Kit; Complement component 4B ELISA Kit; CPAMD3 ELISA Kit
缩写：C4b
Uniprot No.：P0C0L5
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human C4b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 84 | ||||
| Range % | 80-94 | |||||
| 1:400 | Average % | 97 | ||||
| Range % | 91-102 | |||||
| 1:800 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:1600 | Average % | 92 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of human C4b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-98 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.5662.3662.4662.3735002.0311.9311.9811.8882501.4091.3191.3641.2711250.8670.8540.8610.76862.50.5240.5140.5190.42631.20.3450.3250.3350.24215.60.2130.2030.2080.11500.0940.0910.093 | ||||||
货期：3-5 working days
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