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Human Interleukin 13,IL-13 ELISA KIT

  • 中文名： 人白介素13(IL-13)ELISA Kit
货号: IPD-E04601h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人白介素13(IL-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL13含量。IL13是重要的细胞因子靶点。它在免疫调节和炎症反应中发挥关键作用，可调节Th2细胞介导的免疫反应，促进IgE产生。研究发现其与哮喘、特应性皮炎等疾病密切相关，针对IL13开发药物以阻断其信号通路成为研究方向。试剂盒检测范围为0.39 ng/ml-25 ng/ml.，灵敏度为0.09 ng/ml。可广泛应用于免疫学基础研究、炎症性疾病模型构建、药物作用机制探索等场景，为体外实验中IL - 13的动态监测提供稳定检测平台。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Allergic rhinitis ELISA Kit; ALRH ELISA Kit; BHR 1 ELISA Kit; BHR1 ELISA Kit; Bronchial hyperresponsiveness 1 (bronchial asthma) ELISA Kit; IL 13 ELISA Kit; IL-13 ELISA Kit; Il13 ELISA Kit; IL13_HUMAN ELISA Kit; Interleukin 13 ELISA Kit; Interleukin-13 ELISA Kit; interleukin13 ELISA Kit; MGC116786 ELISA Kit; MGC116788 ELISA Kit; MGC116789 ELISA Kit; NC 30 ELISA Kit; NC30 ELISA Kit; P 600 ELISA Kit; P600 ELISA Kit
  • 缩写：
    IL13
  • Uniprot No.：
    P35225
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.39 ng/ml-25 ng/ml.
  • 灵敏度：
    0.09 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    88


    Range %

    83-92


    1:2

    Average %

    101


    Range %

    95-105


    1:4

    Average %

    103


    Range %

    99-105


    1:8

    Average %

    94


    Range %

    90-97


  • 回收率：

    The recovery of human IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    92-97


    EDTA plasma (n=4)

    96

    92-99


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    25

    2.612

    2.554

    2.583

    2.479


    12.5

    2.088

    2.020

    2.054

    1.950


    6.25

    1.231

    1.268

    1.250

    1.146


    3.125

    0.653

    0.607

    0.630

    0.526


    1.56

    0.407

    0.401

    0.404

    0.300


    0.78

    0.235

    0.228

    0.232

    0.128


    0.39

    0.169

    0.159

    0.164

    0.060


    0

    0.105

    0.103

    0.104




  • 货期：
    3-5 working days

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