首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human Interleukin 17A (IL-17A/IL-17) ELISA Kit

  • 中文名： 人白介素17A(IL-17A/IL-17)ELISA Kit
货号: IPD-E12819h
Price： ￥2500
数量：
大包装询价

产品详情

  • 产品描述：
    人白介素17A(IL-17A/IL-17)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL17A含量。IL17A是一种由Th17细胞等分泌的促炎细胞因子。其背景在于它在自身免疫疾病等炎症相关疾病中起重要作用。研究机制上，它能通过与受体结合，激活下游信号通路，诱导炎性介质的产生，从而参与免疫反应，成为治疗相关疾病的潜在靶点。试剂盒检测范围为62.5 pg/ml- 4000 pg/ml，灵敏度为15.6 pg/ml。该产品可广泛用于免疫学、细胞生物学等领域的基础研究，例如炎症反应机制探索、药物干预效果评估或疾病动物模型中细胞因子表达动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CTLA 8 ELISA Kit; CTLA-8 ELISA Kit; CTLA8 ELISA Kit; cytotoxic T lymphocyte associated antigen 8 ELISA Kit; Cytotoxic T lymphocyte associated protein 8 ELISA Kit; Cytotoxic T lymphocyte associated serine esterase 8 ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit; IL 17 ELISA Kit; IL 17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit; IL17 ELISA Kit; IL17_HUMAN ELISA Kit; Il17a ELISA Kit; Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) ELISA Kit; interleukin 17A ELISA Kit; Interleukin-17A ELISA Kit; interleukin17 ELISA Kit; Interleukin17A ELISA Kit; OTTHUMP00000016597 ELISA Kit; OTTMUSP00000046003 ELISA Kit
  • 缩写：
    IL17A
  • Uniprot No.：
    Q16552
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    62.5 pg/ml - 4000 pg/ml
  • 灵敏度：
    15.6 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-17A/IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    104


    Range %

    95-112


    1:2

    Average %

    99


    Range %

    94-105


    1:4

    Average %

    97


    Range %

    92-109


    1:8

    Average %

    93


    Range %

    86-99


  • 回收率：

    The recovery of human IL-17A/IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    89-102


    EDTA plasma (n=4)

    100

    94-108


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    4000

    2.660

    2.634

    2.647

    2.563


    2000

    1.951

    1.867

    1.909

    1.825


    1000

    1.306

    1.328

    1.317

    1.233


    500

    0.766

    0.729

    0.747

    0.663


    250

    0.432

    0.421

    0.426

    0.342


    125

    0.270

    0.253

    0.261

    0.177


    62.5

    0.175

    0.184

    0.180

    0.096


    0

    0.086

    0.082

    0.084




  • 货期：
    3-5 working days

客户数据及评论

折叠内容

大包装询价

×