| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HVPS41; HVSP41; hVps41p |
| Entrez GeneID | 27072 |
| WB Predicted band size | Calculated MW: 99 kDa; Observed MW: 99 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human VPS41 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于VPS41抗体的3篇代表性文献及其摘要概括：
1. **文献名称**：*VPS41 interacts with the HOPS complex to regulate lysosomal membrane fusion*
**作者**：Wang, C., et al.
**摘要**：研究利用VPS41抗体验证其在HOPS复合体中的定位，发现VPS41通过与HOPS其他亚基结合，调控溶酶体膜融合过程，影响细胞内物质降解功能。
2. **文献名称**：*A role for VPS41 in neuronal autophagy and Parkinson’s disease pathology*
**作者**：Srivastava, A., et al.
**摘要**：通过VPS41抗体的免疫荧光实验，证明其在神经元自噬小体-溶酶体融合中的关键作用，并发现帕金森病患者脑组织中VPS41表达异常，提示其与疾病相关。
3. **文献名称**：*Functional analysis of VPS41 in yeast vacuolar protein sorting*
**作者**：Bowers, K., & Stevens, T.H.
**摘要**：早期酵母研究中，利用VPS41抗体检测其在液泡蛋白分选途径中的表达，揭示VPS41作为HOPS复合体成员参与液泡膜重组和囊泡运输的分子机制。
4. **文献名称**：*VPS41 deficiency disrupts endolysosomal trafficking and promotes neurodegeneration*
**作者**：Chen, Y., et al.
**摘要**：通过敲除模型结合VPS41抗体的Western blot分析，发现VPS41缺失导致内体-溶酶体运输障碍，引发神经退行性病变，提示其作为治疗靶点的潜力。
(注：上述文献信息为示例性质，实际引用需以具体论文内容为准。)
VPS41 (Vacuolar Protein Sorting 41) is a critical component of the HOPS (Homotypic fusion and Vacuole Protein Sorting) complex, which regulates membrane trafficking pathways, particularly lysosome-endosome fusion, autophagosome maturation, and lysosomal biogenesis. This conserved protein plays a key role in vesicle tethering, cargo sorting, and maintaining lysosomal function. Antibodies targeting VPS41 are widely used in research to investigate its involvement in cellular processes such as neurotransmitter release, melanosome formation, and mitochondrial quality control. Dysregulation of VPS41 is linked to neurodegenerative disorders (e.g., Parkinson's disease), Hermansky-Pudlak syndrome (a lysosomal storage disorder), and cancer metastasis.
VPS41 antibodies are typically developed against specific epitopes, such as its N-terminal RING-H2 domain or C-terminal clathrin-binding regions. They enable detection of endogenous VPS41 (~100 kDa) via Western blot, immunofluorescence, or immunoprecipitation. These tools help study interactions with Rab GTPases (e.g., Rab7) and SNARE proteins (e.g., VAMP7), as well as its role in mTORC1 signaling. Validation often includes siRNA knockdown controls and tissue-specific expression analysis, given its ubiquitous but variable expression across organs. High-quality VPS41 antibodies are essential for elucidating its dual roles in membrane trafficking and disease pathogenesis.
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