| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | VIL; VIL1; Villin1 |
| Entrez GeneID | 7429 |
| WB Predicted band size | Calculated MW: 93 kDa; Observed MW: 93 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human Villin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于Villin抗体的代表性文献概览：
1. **文献名称**: *"Villin immunohistochemistry is a reliable method for diagnosing microvillus inclusion disease"*
**作者**: Wiegerinck CL, et al.
**摘要**: 研究验证了Villin抗体在免疫组化中的特异性，证实其可用于诊断微绒毛包涵体病(MVID)，通过检测小肠活检样本中微绒毛结构的异常分布。
2. **文献名称**: *"Utility of Villin immunohistochemistry in differentiating primary ovarian carcinoma from metastatic colorectal carcinoma"*
**作者**: Vakiani E, et al.
**摘要**: 探讨Villin抗体在鉴别卵巢原发癌与转移性结直肠癌中的应用，发现Villin阳性表达支持结直肠来源，辅助临床病理鉴别诊断。
3. **文献名称**: *"Villin expression in renal cell carcinoma and its correlation with tumor grade and patient survival"*
**作者**: Lin F, et al.
**摘要**: 分析Villin在肾细胞癌中的表达模式，发现其与肿瘤分级和患者预后相关，提示其可能作为潜在生物标志物。
4. **文献名称**: *"Development of a monoclonal antibody specific to human Villin and its application in gastrointestinal cancer research"*
**作者**: Robine S, et al.
**摘要**: 报道一种高特异性抗人Villin单克隆抗体的开发，并验证其在胃肠道癌症研究中对上皮细胞极性和分化的标记作用。
这些文献覆盖了Villin抗体在疾病诊断、肿瘤鉴别及基础研究中的关键应用。如需具体文章链接或发表年份，可进一步补充检索条件。
Villin antibodies are essential tools in biomedical research and diagnostic pathology, primarily targeting villin, a cytoskeletal protein associated with microvilli in epithelial cells. Villin, encoded by the *VIL1* gene, belongs to the gelsolin family of actin-binding proteins and plays a role in maintaining cell polarity, microvillar structure, and actin remodeling. It is predominantly expressed in absorptive epithelial cells of the gastrointestinal tract (e.g., small intestine, colon), renal proximal tubules, and biliary ducts.
In diagnostics, villin antibodies are widely used as immunohistochemical markers to identify tumors of intestinal origin. They help differentiate adenocarcinomas (e.g., colorectal, pancreaticobiliary) from non-epithelial or neuroendocrine neoplasms. Villin positivity typically manifests as cytoplasmic or membranous staining, with brush border patterns in well-differentiated tumors. Its expression is often retained in metastatic lesions, aiding in tracing primary tumor sites.
Research applications include studying epithelial cell differentiation, cell migration, and microvilli formation. Villin’s role in actin dynamics also links it to pathological processes like cancer metastasis. Commercially available antibodies are typically monoclonal (e.g., clone ID2C3) and validated for formalin-fixed tissues. However, specificity varies; cross-reactivity with non-gastrointestinal tumors (e.g., ovarian, lung) necessitates cautious interpretation alongside other markers like CDX2 or cytokeratins. Overall, villin antibodies remain pivotal in both understanding epithelial biology and improving cancer diagnostics.
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