| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | VCL; Vinculin; Metavinculin |
| Entrez GeneID | 7414 |
| WB Predicted band size | Calculated MW: 124 kDa; Observed MW: 124 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human Vinculin |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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1. **"Vinculin regulates cell-surface E-cadherin expression by binding to β-catenin"**
*作者：Peng X 等*
摘要：研究揭示了Vinculin通过结合β-catenin调控细胞表面E-cadherin的稳定性，影响上皮细胞间粘附。实验利用特异性Vinculin抗体进行免疫共沉淀，证实其在细胞连接处的功能关联。
2. **"Structural basis of vinculin activation by talin"**
*作者：Bakolitsa C 等*
摘要：通过X射线晶体学解析Vinculin与Talin结合的活性构象，阐明其激活机制。研究使用Vinculin抗体验证构象变化，为理解细胞力学信号转导提供结构基础。
3. **"Vinculin antibody exacerbates experimental colitis by disrupting intestinal barrier function"**
*作者：Chen Y 等*
摘要：发现抗Vinculin抗体会破坏小鼠肠道上皮屏障完整性，加剧结肠炎。研究提示针对Vinculin的自身抗体可能参与炎症性肠病的病理过程。
4. **"Antibody-based profiling of vinculin phosphorylation reveals distinct roles in cell migration"**
*作者：Saunders RM 等*
摘要：开发磷酸化特异性Vinculin抗体，揭示不同位点磷酸化(如Tyr100/106)对细胞迁移的差异调控，为肿瘤转移研究提供新型检测工具。
Vinculin is a cytoskeletal protein that plays a critical role in cell-cell and cell-matrix adhesion by linking integrins or cadherins to the actin cytoskeleton. It is enriched at focal adhesions and adherens junctions, where it regulates cell adhesion, migration, and mechanotransduction signaling. Vinculin exists in an autoinhibited conformation; upon activation by binding partners like talin or α-catenin, it undergoes conformational changes to mediate cytoskeletal remodeling.
Antibodies targeting vinculin are widely used to study its localization, expression, and function in cellular processes. They are essential tools in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Commercially available vinculin antibodies often recognize conserved epitopes across species (human, mouse, rat) and can distinguish between full-length vinculin (~124 kDa) and its splice variant metavinculin (~150 kDa). Monoclonal antibodies (e.g., clone hVIN-1) offer high specificity, while polyclonal antibodies may detect broader isoforms.
Vinculin dysregulation is implicated in cancer metastasis, cardiovascular diseases, and wound healing, making its antibodies valuable in biomedical research. Researchers should validate antibody specificity using knockout cell lines or siRNA knockdown, as cross-reactivity with homologous proteins (e.g., metavinculin in cardiac tissue) may occur. Proper controls, including tissue or cell lysates with known vinculin expression, are recommended to ensure experimental accuracy.
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