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产品描述：牛脂多糖结合蛋白(LBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LBP含量。LBP即脂多糖结合蛋白，它在人体免疫防御中扮演重要角色，能与脂多糖结合。研究机制方面，LBP可增强单核细胞、巨噬细胞等对脂多糖的识别和反应，激活免疫信号通路，在炎症反应、抗感染等过程发挥作用，对它的研究有助于深入了解免疫机制。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于牛类感染性疾病模型建立、炎症相关机制研究及LPS介导的病理学分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LBPLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
缩写：LBP
Uniprot No.：Q2TBI0
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 84-102 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 82-95 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 90-107 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of bovine LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 10002.1262.1502.1382.002 5001.6111.6231.6171.481 2501.1421.1581.1501.014 1250.7970.7800.7890.653 62.50.4630.4510.4570.321 31.250.3400.3340.3370.201 15.60.2710.2750.2730.137 00.1350.1360.136 | ||||||
货期：3-5 working days
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