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产品描述：牛生长激素 (GH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GH1含量。GH1是一个重要靶点。它与生长激素的合成、分泌相关，在人体生长发育进程中扮演关键角色。科研人员聚焦其作用机制，探索通过调控GH1来改善生长发育异常疾病，为相关病症的治疗提供了新的方向与思路。试剂盒检测范围为1.25 ng/mL-50 ng/mL，适用于科研领域研究GH在牛生理调控、营养代谢、繁殖周期中的表达规律，也可用于评估饲养管理方案或外源激素干预对GH分泌的影响，为畜牧生产研究提供可靠数据支持。适用于实验室常规检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GH1 ELISA Kit; GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
缩写：GH1
Uniprot No.：P01246
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-50 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 101 | ||||
| Range % | 98-105 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 90-96 | |||||
回收率：
| The recovery of bovine GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-99 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 500.3320.3310.332 250.5380.5080.523 11.50.7680.7350.752 3.751.0861.0991.093 1.251.6321.6571.645 02.1312.1682.150 | ||||||
货期：3-5 working days
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