首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠神经肽Y(NPY)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NPY含量。NPY即神经肽Y，是一种广泛分布于中枢和外周神经系统的神经肽。它参与多种生理功能调节，如摄食、能量代谢、心血管活动等。研究机制方面，主要通过与不同亚型受体结合，激活下游信号通路来发挥作用，在相关疾病治疗研究中是潜在靶点。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于神经生物学研究、代谢调节机制探索、药物干预效果评价等科研领域。支持大鼠模型中的NPY动态监测，例如神经退行性疾病模型、肥胖症研究或应激反应实验中的生物标志物分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NPY
Uniprot No.：P07808
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-95 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of rat NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-99 | ||||
| EDTA plasma (n=4) | 105 | 100-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.4872.3852.4362.290501.6291.5271.5781.432250.9160.9370.9270.78112.50.5740.5650.5700.4246.250.3750.3860.3810.2353.120.2610.2500.2560.1101.560.1860.1820.1840.03800.1460.1450.146 | ||||||
货期：3-5 working days
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