首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠黏多糖/糖胺聚糖酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、尿液样本中的GAGs含量。GAGs即糖胺聚糖，在人体组织细胞中广泛分布，参与众多生理病理过程。研究发现，它与细胞黏附、迁移、增殖等活动密切相关，还在肿瘤发生发展、炎症反应等机制里发挥作用，是疾病治疗靶点以及药物研发的重要研究对象。试剂盒检测范围为6.25 ng/mL-100 ng/mL，在代谢性疾病模型研究、药物干预效果评估及组织修复机制探索中具有重要应用价值；可广泛应用于大鼠疾病模型（如黏多糖贮积症、关节炎等）的病理机制研究、代谢调控实验及生物标志物筛选等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GAGs
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, urine
检测范围：6.25 ng/mL-100 ng/mL
灵敏度：3.125 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat glycosaminoglycan in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-88 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 83-94 | |||||
回收率：
| The recovery of rat glycosaminoglycan spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average1000.0810.0860.084500.1950.2010.198250.4900.5100.50012.50.9250.9020.9146.251.6881.7051.69702.5872.6122.600 | ||||||
货期：3-5 working days
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