首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠基质金属蛋白酶8/中性粒细胞胶原酶(MMP-8/Collagenase II)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP8含量。MMP8 即基质金属蛋白酶 8，在生理上参与组织重塑等过程。其异常表达与多种疾病相关，如炎症、肿瘤等。研究机制主要围绕其在疾病发生发展中对细胞外基质的降解作用，以及与其他信号通路的交互，为相关疾病治疗提供潜在靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，可满足体外实验或动物模型中MMP - 8表达水平的动态监测需求，例如关节炎模型、肺部炎症研究或创伤修复机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp8Neutrophil collagenase ELISA Kit; EC 3.4.24.34 ELISA Kit; Matrix metalloproteinase-8 ELISA Kit; MMP-8 ELISA Kit
缩写：MMP8
Uniprot No.：O88766
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-108 | |||||
回收率：
| The recovery of rat MMP8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 84 | 80-88 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6202.4592.5402.363102.0562.1462.1011.92451.3831.4321.4081.2312.50.8250.8010.8130.6361.250.5090.5180.5140.3370.6250.4170.4050.4110.2340.3120.2510.2570.2540.07700.1800.1730.177 | ||||||
货期：3-5 working days
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