首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠基质金属蛋白酶14(MMP-14)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP14含量。MMP14 即基质金属蛋白酶 14，在细胞外基质重塑等生理过程中发挥重要作用。其异常表达与肿瘤生长、侵袭和转移密切相关。研究聚焦于其催化机制和调控网络，旨在揭示其在疾病发生发展中的作用，为开发针对性治疗策略提供依据。试剂盒检测范围为46.88 pg/mL-3000 pg/mL，适用于探索大鼠模型中MMP-14在疾病发生发展中的作用机制，例如评估肿瘤微环境调控、组织修复异常或病理状态下的酶活性变化。可为体外实验提供定量数据支持，助力分子机制研究与药物开发。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MMP14
Uniprot No.：Q10739
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：46.88 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 88-95 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 90-102 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 95-107 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-105 | ||||||
回收率：
| The recovery of rat MMP-14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-96 | |||||
| EDTA plasma (n=4) | 98 | 95-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 30002.2882.2892.2892.092 15001.7361.7521.7441.547 7501.1181.0971.1080.911 3750.6740.7270.7010.504 187.50.4550.4330.4440.247 93.750.3360.3310.3340.137 46.880.2650.2560.2610.064 00.2050.1890.197 | |||||||
货期：3-5 working days
×
