首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠甘丙肽/甘丙素(GAL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GAL含量。试剂盒检测范围为4.7 pg/mL-300 pg/mL，适用于动物模型研究中的神经调控机制探索、代谢相关疾病（如肥胖、糖尿病）的分子机制分析，以及神经系统疾病（如阿尔茨海默病、帕金森病）相关生物标志物的定量检测；为神经科学、代谢研究与药效评估提供可靠的工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gal ELISA Kit; Galn ELISA Kit; Galanin peptides [Cleaved into: Galanin; Galanin message-associated peptide ELISA Kit; GMAP)] ELISA Kit
缩写：GAL
Uniprot No.：P10683
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 pg/mL-300 pg/mL
灵敏度：1.17 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-105 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-110 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat GAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-99 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected3002.6022.5512.5772.4531501.8521.8921.8721.748751.3141.3251.3201.19637.50.8690.8450.8570.73318.750.5110.5360.5240.4009.40.3060.2880.2970.1734.70.2320.2190.2260.10200.1270.1210.124 | ||||||
货期：3-5 working days
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