首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠B细胞淋巴瘤因子2(Bcl-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BCL2含量。BCL2是一种凋亡抑制因子，主要位于核膜、内质网膜和线粒体外膜，通过抑制线粒体细胞色素C的释放和结合凋亡激活因子来抑制caspase活性，从而抑制细胞凋亡。其研究机制涉及调控细胞凋亡和肿瘤发生，是癌症治疗的重要靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，广泛应用于肿瘤模型建立、细胞凋亡机制研究、神经退行性疾病动物实验及药物干预效果评价等科研领域，尤其适用于比较不同处理条件下（如基因敲除、化合物处理）生物样本中Bcl - 2的表达差异。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bcl2; Bcl-2; Apoptosis regulator Bcl-2
缩写：BCL2
Uniprot No.：P49950
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat BCL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 86-102 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 82-104 | |||||
回收率：
| The recovery of rat BCL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-100 | ||||
| EDTA plasma (n=4) | 95 | 87-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.7452.7682.7572.572 102.1762.1652.1711.986 51.4851.4631.4741.289 2.50.8960.8640.8800.695 1.250.5690.5430.5560.371 0.6250.4250.4390.4320.247 0.3120.2960.2840.2900.105 00.1850.1840.185 | ||||||
货期：3-5 working days
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