首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠低密度脂蛋白胆固醇(LDL-C)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LDL-C含量。LDL-C（低密度脂蛋白胆固醇）作为降脂治疗的重要靶点，其研究背景源于其对心血管疾病风险的预测作用。研究机制主要围绕LDL-C在动脉粥样硬化形成中的作用，通过降低LDL-C水平来预防心血管疾病。试剂盒检测范围为125 ng/mL-8000 ng/mL，适用于实验室开展动脉粥样硬化模型评估、脂质代谢通路研究、降脂药物筛选等科研项目，为心血管疾病基础研究及营养学实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LDL-C
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：125 ng/mL-8000 ng/mL
灵敏度：62.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 91-97 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of rat LDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-99 | ||||
| EDTA plasma (n=4) | 103 | 98-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 80000.0890.0880.089 40000.1820.1780.180 20000.3760.3690.373 10000.6300.6420.636 5001.1301.1461.138 2501.5281.5161.522 1251.9141.8101.862 02.2672.1692.218 | ||||||
货期：3-5 working days
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