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产品描述：人T细胞表面糖蛋白CD3ζ链(CD247)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD247含量。CD247 是一种重要靶点。它主要存在于 T 细胞等表面，参与免疫信号传导。相关研究聚焦于其在免疫激活机制中的作用，通过调节它有望增强机体免疫能力，为肿瘤、自身免疫病等疾病的免疫治疗提供新途径和策略。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于评估T细胞功能状态、免疫调控机制研究、药物开发中免疫调节效应验证等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD247; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247
缩写：CD247
Uniprot No.：P20963
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD247 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 106 | ||||
| Range % | 100-115 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human CD247 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 93-102 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.1682.1312.1501.94651.5701.5361.5531.3492.51.1441.1261.1350.9311.250.7750.8150.7950.5910.6250.4820.4980.4900.2860.3120.3530.3640.3590.1550.1560.2560.2650.2610.05700.1990.2090.204 | ||||||
货期：3-5 working days
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