| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CEDNIK; SNAP-29 |
| Entrez GeneID | 9342 |
| WB Predicted band size | Calculated MW: 29 kDa; Observed MW: 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human SNAP29 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3-4篇关于SNAP29抗体的参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**: *SNAP29 mediates assembly of a subset of SNARE proteins required for human epidermal differentiation*
**作者**: Sprecher E. et al. (2005)
**摘要**: 研究通过免疫印迹和免疫组化分析，发现SNAP29在CEDNIK综合征(神经皮肤发育障碍)患者中表达显著降低。实验使用SNAP29抗体验证了患者皮肤细胞中蛋白水平的减少，表明其突变导致表皮分化异常。
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2. **文献名称**: *The SNARE protein SNAP29 regulates autophagosome maturation in mammalian cells*
**作者**: Moreau K. et al. (2014)
**摘要**: 本研究利用SNAP29抗体进行免疫荧光共定位实验，证明SNAP29通过与STX17和VAMP8等SNARE蛋白结合，调控自噬体-溶酶体融合过程。抗体检测显示SNAP29缺失导致自噬体积累，提示其在自噬成熟中的关键作用。
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3. **文献名称**: *Structural and functional analysis of the human SNARE protein SNAP29 in membrane fusion*
**作者**: Xu H. et al. (2010)
**摘要**: 通过免疫沉淀和Western blot实验(使用SNAP29特异性抗体)，揭示了SNAP29在SNARE复合体中的结构特征及其在细胞内运输中的功能。研究表明，SNAP29通过结合Syntaxin和VAMP家族成员介导膜融合。
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4. **文献名称**: *SNAP29 controls the recruitment of Rab GTPases to regulate secretory granule maturation*
**作者**: Suga K. et al. (2015)
**摘要**: 实验通过RNA干扰结合SNAP29抗体检测，发现SNAP29通过招募Rab3和Rab27等GTP酶调控分泌颗粒的成熟。抗体验证了SNAP29在分泌细胞中的动态定位变化，支持其在胞吐作用中的调控机制。
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以上文献均涉及SNAP29抗体的实验应用，涵盖疾病机制、自噬调控、结构功能及分泌途径等领域。如需具体DOI或期刊信息，可进一步补充。
**Background of SNAP29 Antibody**
SNAP29 (Synaptosome-Associated Protein 29 kDa) is a member of the SNARE (Soluble NSF Attachment Protein Receptor) family, which plays a critical role in intracellular membrane fusion and vesicle trafficking. Unlike other SNARE proteins, SNAP29 lacks a transmembrane domain and is thought to function as a regulatory SNARE, mediating interactions between target membranes and secretory vesicles. It is ubiquitously expressed and participates in diverse cellular processes, including autophagy, endosomal sorting, and exocytosis.
Antibodies targeting SNAP29 are essential tools for studying its expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect SNAP29 in various cell types and tissues. Research has linked SNAP29 dysregulation to human diseases, such as CEDNIK syndrome (a neurocutaneous disorder caused by SNAP29 mutations), cancer, and immune deficiencies. Studies also explore its interactions with partners like STX4 (syntaxin-4) and VAMP8. which are critical for membrane fusion events.
SNAP29 antibodies often recognize conserved epitopes, such as its two SNARE domains or the linker region, aiding in functional studies. Their specificity is validated using knockout controls or siRNA-mediated silencing. These reagents contribute to understanding SNAP29's role in cellular homeostasis, disease mechanisms, and potential therapeutic targeting.
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