首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠血纤蛋白原(Fbg)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Fbg含量。Fbg即纤维蛋白原，是血浆中的凝血因子。其在凝血过程中起关键作用，在凝血酶作用下转变为纤维蛋白参与血栓形成。研究多围绕其与血栓性疾病关联展开，探索抑制其功能或生成的机制，以研发抗血栓药物。试剂盒检测范围为0.312 μg/mL-20 μg/mL，适用于凝血机制研究、肝损伤模型评估或炎症相关疾病模型的科研场景，例如通过检测血浆样本分析凝血功能异常，或通过组织匀浆检测探究局部炎症反应中Fbg的调控作用。为心血管、肝脏及免疫相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Fbg
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 μg/mL-20 μg/mL
灵敏度：0.156 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 99 | ||||
| Range % | 91-103 | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 82-94 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 91-106 | |||||
| 1:800 | Average % | 101 | ||||
| Range % | 91-105 | |||||
回收率：
| The recovery of rat Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 93-104 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average200.0850.0900.088100.1840.1780.18150.3860.4040.3952.50.6480.6790.6641.251.1921.1771.1850.6251.5181.5421.5300.3121.6871.6381.66302.1692.0372.103 | ||||||
货期：3-5 working days
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