首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠同型半胱氨酸(Hcy)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Hcy含量。同型半胱氨酸（Hcy）是蛋氨酸代谢的中间产物。高Hcy水平是心血管疾病、神经系统疾病等多种疾病的独立危险因素。其研究机制主要围绕Hcy诱导氧化应激、损伤血管内皮细胞、影响细胞甲基化反应等方面，以揭示其在疾病发生发展中的作用。试剂盒检测范围为15.6 pmol/mL-1000 pmol/mL，适用于科研场景中探究Hcy在心血管疾病模型、神经损伤研究或营养代谢实验中的动态变化，也可用于药物干预或基因修饰后Hcy代谢通路的机制研究，为相关疾病模型构建及代谢调控分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Hcy
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：15.6 pmol/mL-1000 pmol/mL
灵敏度：3.9 pmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:40 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:80 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:160 | Average % | 93 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 82-95 | ||||
| EDTA plasma (n=4) | 99 | 96-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pmol/mlOD1OD2AverageCorrected10002.7492.8002.7752.5785002.0322.1132.0731.8762501.3651.3861.3761.1791250.8480.9040.8760.67962.50.5430.5630.5530.35631.20.3720.4030.3880.19115.60.2780.3020.2900.09300.1980.1950.197 | ||||||
货期：3-5 working days
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