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产品描述：猪生饥饿激素(GHRL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GHRL含量。GHRL即胃饥饿素，是胃分泌的一种肽。它能调节食欲和能量稳态，与肥胖、糖尿病等代谢疾病相关。研究机制主要围绕其与受体的结合，通过信号传导调节神经内分泌，影响进食行为和代谢过程，为相关疾病治疗提供潜在靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，本试剂盒适用于科研场景中GHRL相关基础研究，例如探索营养干预、代谢性疾病模型或生理周期变化对GHRL表达的影响，满足能量代谢机制、内分泌调控通路等领域的科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GHRLAppetite-regulating hormone ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin] ELISA Kit
缩写：GHRL
Uniprot No.：Q9GKY5
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:4 | Average % | 106 | ||||
| Range % | 100-114 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of pig GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 88 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.4812.5832.5322.38220001.8731.9041.8891.73910001.2931.2211.2571.1075000.7340.7080.7210.5712500.4270.4620.4450.2951250.3220.3020.3120.16262.50.2500.2590.2550.10500.1540.1460.150 | ||||||
货期：3-5 working days
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