首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠高半胱氨酸(Hcy)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Hcy含量。Hcy即同型半胱氨酸，是蛋氨酸代谢过程中的中间产物。其水平异常升高是心血管疾病等多种疾病的独立危险因素。研究机制方面，高Hcy可损伤血管内皮细胞，影响脂质代谢，还能促进血栓形成，干扰一氧化氮合成，从而引发一系列病理变化。试剂盒检测范围为4.7 nmol/mL-300 nmol/mL，适用于小鼠模型中的代谢功能评估、疾病进程监测或药物干预效果分析，例如在动脉粥样硬化、阿尔茨海默病等研究中动态观察Hcy水平变化，或在营养干预实验中探究维生素B族对Hcy代谢的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：Hcy
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 nmol/mL-300 nmol/mL
灵敏度：1.1 nmol/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 86-96 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 95-107 | |||||
回收率：
| The recovery of mouse Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| nmol/mlOD1OD2AverageCorrected 3002.4372.3522.3952.222 1502.0752.0362.0561.883 751.6741.6041.6391.466 37.51.0791.1321.1060.933 18.750.6210.5970.6090.436 9.40.4160.4590.4380.265 4.70.2970.2890.2930.120 00.1790.1670.173 | ||||||
货期：3-5 working days
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