首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠胶原吡啶交联(PYD)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的PYD含量。小鼠胶原吡啶交联（PYD）是一种通过吡啶交联剂对胶原蛋白进行交联的技术，旨在改善胶原蛋白的物理和生物性能。该技术通过交联反应增加胶原蛋白的稳定性和机械强度，广泛应用于组织工程和再生医学领域，以促进组织修复和再生。试剂盒检测范围为50 ng/mL-800 ng/mL，适用于骨代谢机制研究、骨质疏松动物模型评估、抗骨吸收药物开发等科研领域，为骨代谢相关基础研究提供高效可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PYD
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：50 ng/mL-800 ng/mL
灵敏度：50 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 92-106 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 83-99 | |||||
回收率：
| The recovery of mouse PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 88-97 | ||||
| EDTA plasma (n=4) | 93 | 91-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 8000.1470.1450.146 4000.2410.2540.248 2000.5340.5540.544 1001.1021.1391.121 502.3182.2782.298 | ||||||
货期：3-5 working days
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