首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠髓鞘碱性蛋白(MBP)抗体酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的MBP Ab含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于神经退行性疾病模型研究、神经损伤修复机制探索以及药物开发过程中MBP生物标志物的动态监测。支持科研人员高效开展神经科学基础研究、病理模型评估或神经保护剂筛选等实验，为揭示MBP在神经生物学中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MBP Ab
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MBP antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 85-97 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 89-99 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 81-97 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 91-100 | ||||||
回收率：
| The recovery of rat MBP antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-102 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 202.7862.7632.7752.538 102.1052.0992.1021.865 51.3461.3181.3321.095 2.50.8050.7940.8000.563 1.250.6010.5950.5980.361 0.6250.4520.4490.4510.214 0.3120.3530.3560.3550.118 00.2350.2390.237 | |||||||
货期：3-5 working days
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