首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠抗甲状腺过氧化物酶抗体(TPO-Ab)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的TPO Ab含量。TPO Ab即抗血小板生成素抗体。其背景在于它可能参与多种疾病进程，尤其是与血小板生成相关疾病。研究机制上，TPO Ab会与血小板生成素结合，干扰正常的血小板生成信号传导通路，进而影响血小板的生成数量和功能。试剂盒检测范围为5 IU/mL-80 IU/mL，适用于科研场景下甲状腺疾病发病机制研究、药物干预效果评估或动物模型中的免疫状态分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：TPO Ab
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：5 IU/mL-80 IU/mL
灵敏度：5 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-TPO antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 86-101 | ||||||
| 1:2 | Average % | 88 | |||||
| Range % | 83-98 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 89-100 | ||||||
回收率：
| The recovery of rat anti-TPO antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 92-99 | |||||
| EDTA plasma (n=4) | 95 | 90-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| IU/mlOD1OD2Average 800.1710.1690.17 400.2850.2760.281 200.4950.5050.5 101.0371.0141.026 52.0322.0662.049 | |||||||
货期：3-5 working days
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