首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠谷氨酸脱羧酶自身抗体IgG(GAD-Ab-IgG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的GAD auto-Ab (IgG)含量。GAD auto - Ab (IgG)即谷氨酸脱羧酶自身抗体（IgG 型），在自身免疫性疾病背景中有重要意义，如在 1 型糖尿病等疾病中常出现。它会攻击表达 GAD 的细胞，破坏细胞功能，影响神经递质合成等过程，进而影响机体正常生理活动。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于探索1型糖尿病、神经系统疾病等动物模型中GAD-Ab-IgG的动态变化，为研究自身抗体介导的病理过程提供数据支持。本产品严格限定于科学研究用途，适用于基础医学、免疫学及疾病机制研究等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GAD auto-Ab (IgG)
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GAD autoantibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:5 | Average % | 86 | |||||
| Range % | 81-97 | ||||||
| 1:10 | Average % | 89 | |||||
| Range % | 85-100 | ||||||
| 1:20 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:40 | Average % | 93 | |||||
| Range % | 86-99 | ||||||
回收率：
| The recovery of rat GAD autoantibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 82-98 | |||||
| EDTA plasma (n=4) | 93 | 87-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected5001.8561.8951.8761.7062501.2681.2551.2621.0921250.8840.9020.8930.72362.50.5690.5840.5770.40731.20.3920.4030.3980.22815.60.2870.2920.2900.1207.80.2380.2360.2370.06700.1670.1720.170 | |||||||
货期：3-5 working days
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