首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠髓过氧化物酶特异性抗中性粒细胞胞质抗体酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的MAC Ab含量。小鼠髓过氧化物酶（MPO）特异性抗中性粒细胞胞质抗体（ANCA）与血管炎密切相关，感染可能引发MPO-ANCA产生，进而诱发血管炎。MPO-ANCA通过诱导中性粒细胞活化及形成中性粒细胞外捕获物（NETs）促进ANCA产生，NETs中包含MPO，可能触发机体产生自身抗体导致血管炎。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域探究小鼠模型中MPO - ANCA的动态变化，如评估炎症性疾病进展、自身免疫模型构建或药物干预效果研究。支持科研人员在体外实验中高效获取稳定可靠的实验数据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MAC Ab
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA)in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 83-101 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-106 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 82-101 | |||||
回收率：
| The recovery of mouse myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA)spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-98 | ||||
| EDTA plasma (n=4) | 94 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.4042.4182.4112.297 201.9561.9041.9301.816 101.3521.3771.3651.251 50.9020.9340.9180.804 2.50.5260.5340.5300.416 1.250.3110.3260.3190.205 0.6250.2650.2710.2680.154 00.1120.1150.114 | ||||||
货期：3-5 working days
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