| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ROC1; RNF75; BA554C12.1 |
| Entrez GeneID | 9978 |
| WB Predicted band size | Calculated MW: 12 kDa; Observed MW: 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human ROC1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于RBX1抗体的3篇文献概览，涵盖其功能、结构及疾病关联研究：
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1. **文献名称**: *Structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase complex*
**作者**: Zheng, N., Schulman, B.A., et al.
**摘要**:
该研究解析了Cul1-Rbx1-Skp1-F box复合体的晶体结构，揭示RBX1作为RING结构域蛋白在泛素转移中的关键作用。研究中通过抗体验证RBX1在SCF复合体中的定位及稳定性，为泛素化机制提供了结构基础。
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2. **文献名称**: *NEDD8 links cullin-RING ubiquitin ligase function to the COP9 signalosome*
**作者**: Skaar, J.R., Pagano, M., et al.
**摘要**:
文章探讨NEDD8修饰对CRL复合体活性的调控，发现RBX1是NEDD8结合的关键靶点。通过RBX1抗体进行免疫共沉淀实验，证明其与Cullin蛋白的互作依赖NEDD8化，调控底物泛素化及降解。
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3. **文献名称**: *Overexpression of RBX1 in colorectal cancer associates with tumor progression and poor prognosis*
**作者**: Kim, Y., et al.
**摘要**:
研究利用RBX1抗体对结直肠癌组织进行免疫组化分析，发现RBX1高表达与肿瘤分期、转移及生存率下降显著相关。Western blot验证其通过激活CRL促进致癌蛋白降解，提示RBX1作为潜在治疗靶点。
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这些文献展示了RBX1抗体在结构生物学、分子机制及临床研究中的关键应用。如需具体文献来源，建议通过PubMed或期刊数据库检索标题及作者。
**Background of RBX1 Antibody**
RBX1 (RING-box protein 1), also known as ROC1. is a critical component of the SCF (Skp1-Cul1-F-box protein) E3 ubiquitin ligase complex, which plays a central role in the ubiquitin-proteasome system (UPS). This system regulates protein degradation, a process essential for maintaining cellular homeostasis, cell cycle progression, DNA repair, and signal transduction. RBX1 contains a RING-finger domain that facilitates the recruitment of E2 ubiquitin-conjugating enzymes, enabling the transfer of ubiquitin to substrate proteins targeted for proteasomal degradation.
RBX1 is indispensable for the assembly and activity of Cullin-RING ligases (CRLs), the largest family of E3 ubiquitin ligases. Its interaction with Cullin proteins stabilizes the CRL complex and enhances substrate ubiquitination. Dysregulation of RBX1 has been linked to various diseases, including cancers, where aberrant UPS activity contributes to uncontrolled proliferation, metastasis, or resistance to therapy. For instance, RBX1 overexpression is observed in certain tumors, correlating with poor prognosis.
Antibodies targeting RBX1 are vital tools for studying its expression, localization, and function in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to investigate RBX1’s role in CRL-mediated proteolysis, cellular stress responses, and oncogenic pathways. Additionally, these antibodies aid in exploring therapeutic strategies aimed at modulating UPS activity, such as developing CRL inhibitors. High-quality RBX1 antibodies are characterized by specificity, sensitivity, and compatibility across diverse experimental models, ensuring reliable data in mechanistic and translational research.
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