| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ROCK1; Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated; coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK |
| Entrez GeneID | 6093 |
| WB Predicted band size | Calculated MW: 158 kDa; Observed MW: 158 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human ROCK1 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于ROCK1抗体的文献示例(信息基于公开论文总结，非真实文献，仅供参考格式)：
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1. **文献名称**: *ROCK1-mediated phosphorylation promotes autophagy in cancer cells*
**作者**: Li X, et al.
**摘要**: 研究利用ROCK1特异性抗体发现，ROCK1通过磷酸化ULK1蛋白激活自噬，促进乳腺癌细胞的存活和化疗耐药性，提示ROCK1可作为治疗靶点。
2. **文献名称**: *Development of a high-affinity ROCK1 monoclonal antibody for cardiovascular disease models*
**作者**: Smith J, et al.
**摘要**: 报道了一种新型ROCK1单克隆抗体的开发，验证其特异性并应用于小鼠动脉粥样硬化模型，显示抑制ROCK1可减少血管平滑肌细胞异常迁移。
3. **文献名称**: *ROCK1/2 dual inhibition attenuates fibrosis in pulmonary hypertension*
**作者**: Chen Y, et al.
**摘要**: 通过ROCK1/2抗体对比实验，发现同时抑制ROCK1和ROCK2可协同下调TGF-β信号通路，显著减轻肺纤维化及肺动脉高压病理进展。
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(注：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索关键词“ROCK1 antibody”获取。)
ROCK1 (Rho-associated protein kinase 1) is a serine/threonine kinase that plays a pivotal role in regulating cytoskeletal dynamics, cell adhesion, migration, and apoptosis. It is a downstream effector of the small GTPase RhoA, forming part of the Rho/ROCK signaling pathway implicated in diverse cellular processes. Structurally, ROCK1 contains an N-terminal kinase domain, a central coiled-coil region, and a C-terminal pleckstrin homology (PH) domain. Its activation by RhoA-GTP triggers phosphorylation of substrates like myosin light chain (MLC) and LIM kinase, influencing actomyosin contractility and stress fiber formation.
ROCK1 is widely expressed in various tissues and has been linked to pathophysiological conditions, including cardiovascular diseases (hypertension, atherosclerosis), cancer metastasis, fibrosis, and neurological disorders. Aberrant ROCK1 activity is associated with excessive cell proliferation, inflammation, and tissue remodeling. Research tools like ROCK1 antibodies are essential for detecting protein expression, localization, and activation states in experimental models. These antibodies are commonly used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to study ROCK1's role in disease mechanisms or therapeutic responses. Specific inhibitors (e.g., Y-27632) and genetic knockout models further highlight its functional significance, making ROCK1 a target for drug development in multiple disorders.
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