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Rabbit Monoclonal RPA32 Antibody

  • 中文名： RPA32抗体
  • 别    名： 60S acidic ribosomal protein P1; AA409079; AI325195; AU020965; HSSB; ik:tdsubc_2g1; M(2)21C; MGC137236; OTTHUMP00000004008; p32; p34; RCJMB04_6d17 replication protein A2; 32kDa; REPA 2; REPA1; REPA2; Replication factor A protein 2; Replication protein A 3
货号: IPDX21957
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat,Hamster
IF 1/20 Human,Mouse,Rat,Hamster
IHC 1/50-1/100 Human,Mouse,Rat,Hamster
ICC 技术咨询 Human,Mouse,Rat,Hamster
FCM 咨询技术 Human,Mouse,Rat,Hamster
Elisa 咨询技术 Human,Mouse,Rat,Hamster

产品详情

Aliases60S acidic ribosomal protein P1; AA409079; AI325195; AU020965; HSSB; ik:tdsubc_2g1; M(2)21C; MGC137236; OTTHUMP00000004008; p32; p34; RCJMB04_6d17 replication protein A2; 32kDa; REPA 2; REPA1; REPA2; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A; replication protein A1 (70kD); Replication Protein A2 (32kDa); Replication protein A2 32kD; Replication protein A2 32kDa; Replication protein A2; Replication protein A2; 32kDa; RF A; RF-A protein 2; Rf-A2; RFA; RFA2_HUMAN; RP A; RP-A p32; RP-A p34; RP21C; RPA 2; RPA 32; RPA; RPA2; RPA32; RPA34; RPA70; RpLP1; RpP2; xx:tdsubc_2g1; zgc:109822.
Entrez GeneID6118
WB Predicted band sizeCalculated MW: 29 kDa; Observed MW: 29 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,Hamster
ImmunogenA synthetic peptide of human RPA32/RPA2
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于RPA32抗体的3篇参考文献及其摘要内容概览：

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1. **文献名称**: *RPA subunit phosphorylation facilitates chromatin remodeling and homologous recombination at sites of DNA damage*

**作者**: Binz, S.K., Sheehan, A.M., Wold, M.S.

**摘要**: 研究利用RPA32抗体验证其在DNA损伤位点的招募，发现RPA32的磷酸化修饰通过促进染色质重塑复合体(如Rad51)的募集，增强同源重组修复效率。实验表明RPA32抗体在免疫荧光中特异性标记DNA损伤灶。

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2. **文献名称**: *Coordination of DNA damage responses via the Smc5/Smc6 complex*

**作者**: Sleeth, K.M., Sorensen, C.S., Syljuåsen, R.G. et al.

**摘要**: 通过RPA32抗体进行免疫沉淀和Western blot分析，证明Smc5/Smc6复合物与RPA32在DNA复制应激中的相互作用，调控复制叉稳定性及修复通路选择(如HR或NHEJ)。

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3. **文献名称**: *Phosphorylated RPA32 is a critical marker for activation of the ATR-dependent DNA damage response*

**作者**: Anantha, R.W., Sokolova, E., Borowiec, J.A.

**摘要**: 研究使用RPA32磷酸化特异性抗体，证实RPA32的Ser33位点磷酸化是ATR激酶活化的关键标志，揭示了其在DNA损伤信号转导中的早期调控作用。

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**备注**：以上文献均以RPA32抗体作为核心实验工具，涉及DNA损伤应答、修复机制及蛋白质相互作用研究。如需具体期刊信息或DOI，可进一步补充。

背景信息

The RPA32 antibody is a crucial tool for studying the Replication Protein A (RPA) complex, a single-stranded DNA (ssDNA)-binding protein essential for DNA replication, repair, and recombination. RPA32 (also known as RPA2) is one of three subunits (RPA70. RPA32. RPA14) that form the heterotrimeric RPA complex. It plays a central role in stabilizing ssDNA during replication stress and facilitating DNA damage response signaling. Antibodies targeting RPA32 are widely used to investigate its phosphorylation status, particularly at sites like Ser4/Ser8 and Ser33. which are modified by kinases (ATM, ATR, DNA-PK) during genotoxic stress. These post-translational modifications regulate RPA’s interactions with repair proteins (e.g., RAD51. XPA) and checkpoint activation. Researchers employ RPA32 antibodies in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to explore DNA replication fidelity, replication fork dynamics, and cancer-related genomic instability. Dysregulation of RPA function is linked to diseases like cancer and neurodegenerative disorders, making this antibody valuable in both basic research and clinical studies targeting DNA repair pathways.

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