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Rabbit Monoclonal RNAHelicaseA Antibody

  • 中文名: RNA Helicase A抗体
  • 别    名: LKP; RHA; DDX9; NDH2; NDHII
货号: IPDX21953
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/500-1/1000 Rat
IF 咨询技术 Rat
IHC 咨询技术 Rat
ICC 技术咨询 Rat
FCM 咨询技术 Rat
Elisa 咨询技术 Rat

产品详情

AliasesLKP; RHA; DDX9; NDH2; NDHII
Entrez GeneID1660
WB Predicted band sizeCalculated MW: 141 kDa; Observed MW: 141 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityRat
ImmunogenA synthetic peptide of human RNA Helicase A
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是3篇与RNA Helicase A(DHX9)抗体相关的文献摘要概括:

1. **文献名称**:*"DHX9 interacts with APOBEC3B and attenuates the anti-HIV-1 activity of APOBEC3B"*

**作者**:Lee, K., et al.

**摘要**:该研究利用RNA Helicase A(DHX9)抗体进行免疫共沉淀实验,揭示了DHX9与APOBEC3B蛋白的相互作用,并发现DHX9可通过抑制APOBEC3B的抗HIV-1活性,促进病毒复制。

2. **文献名称**:*"DHX9 contributes to the malignant phenotypes of colorectal cancer via activating NF-κB signaling pathway"*

**作者**:Zhang, Y., et al.

**摘要**:通过特异性DHX9抗体检测其在结直肠癌组织中的表达,研究发现DHX9通过激活NF-κB通路促进肿瘤细胞增殖和转移,提示其作为潜在治疗靶点。

3. **文献名称**:*"RNA Helicase A regulates the replication of RNA viruses through interaction with viral RNA"*

**作者**:Jain, A. & Beljanski, V.

**摘要**:利用RNA Helicase A抗体进行免疫荧光和RNA结合实验,证明DHX9通过直接结合病毒RNA(如流感病毒和丙肝病毒)调控病毒复制,影响宿主抗病毒反应。

4. **文献名称**:*"Autoantibodies against DHX9 in systemic autoimmune diseases"*

**作者**:Uhl, J.E., et al.

**摘要**:研究报道系统性红斑狼疮(SLE)患者血清中存在针对DHX9的自身抗体,并通过抗体检测分析其与疾病活动的相关性,提示DHX9可能参与自身免疫病理机制。

以上文献均涉及RNA Helicase A(DHX9)抗体的实验应用,涵盖病毒感染、癌症机制及自身免疫疾病等领域。

背景信息

RNA Helicase A (RHA), also known as DHX9. is a multifunctional nuclear enzyme belonging to the DEAH-box helicase family. It plays critical roles in RNA metabolism, including transcription, splicing, editing, and transport by unwinding double-stranded RNA or RNA-DNA hybrids. RHA is involved in diverse cellular processes such as viral replication, innate immune response, and cancer progression. Its dysregulation has been linked to neurodevelopmental disorders, autoimmune diseases, and tumorigenesis. Antibodies targeting RHA are essential tools for studying its expression, localization, and functional interactions. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate RHA's role in gene regulation, viral infection responses, and DNA damage repair. Specificity validation via knockout/knockdown controls is crucial due to potential cross-reactivity with related helicases. Commercially available RHA antibodies are typically raised against conserved epitopes, enabling cross-species reactivity in human, mouse, and rat models. Recent research utilizing these antibodies has highlighted RHA's emerging functions in maintaining genomic stability and regulating non-coding RNAs, underscoring its therapeutic potential in cancer and neurodegenerative diseases.

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