首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠抗核抗体IgG酶联免疫试剂盒为捕获法ELISA试剂盒，定量检测血清、血浆样本中的ANA Ab (IgG)含量。ANA Ab (IgG)即抗核抗体IgG型。ANA是一组针对细胞核内多种抗原成分的自身抗体，ANA Ab (IgG)在自身免疫性疾病背景下常出现异常。研究机制上，它可与相应抗原结合，形成免疫复合物，激活补体等，参与免疫损伤过程。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于科研领域中对小鼠自身免疫模型的研究，包括疾病发生机制探索、药物干预效果评估以及免疫调节相关实验。适用于动物实验中的系统性红斑狼疮模型、类风湿性关节炎模型等自身免疫相关课题的数据采集。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：ANA Ab (IgG)
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Capture ELISA
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-nuclear antibody(IgG) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 82-89 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of mouse anti-nuclear antibody(IgG) spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 5002.7512.7522.7522.559 2502.3272.3772.3522.159 1251.7001.6751.6881.495 62.50.9930.9680.9810.788 31.20.6370.6250.6310.438 15.60.4010.4160.4090.216 7.80.2990.2890.2940.101 00.1880.1970.193 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse IgG antibody.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled antigen(100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-conjugateDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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