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产品描述：人精子相关抗原9(SPAG9) 抗体酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的SPAG9 Ab含量。试剂盒检测范围为50 mU/mL-1200 mU/mL，本试剂盒特别适用于生殖生物学基础研究、男性不育相关分子机制探索、生殖系统疾病模型构建等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：SPAG9 Ab
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：50 mU/mL-1200 mU/mL
灵敏度：20 mU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<10% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SPAG9 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human SPAG9 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 86-98 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mU/mlOD1OD2AverageCorrected 12002.0041.9491.9771.930 6001.3341.3181.3261.279 3000.7720.7830.7780.731 1000.3510.3700.3610.314 500.2230.2120.2180.171 00.0470.0460.047 | ||||||
货期：3-5 working days
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