| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BBC3; PUMA; Bcl-2-binding component 3; JFY-1; p53 up-regulated modulator of apoptosis |
| Entrez GeneID | 27113 |
| WB Predicted band size | Calculated MW: 21 kDa; Observed MW: 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human PUMA |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇与PUMA抗体相关的代表性文献(示例为模拟生成内容，实际文献需根据具体数据库查询)：
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1. **文献名称**：*PUMA mediates the apoptotic response to DNA damage in human cells*
**作者**：Yu J, Zhang L
**摘要**：该研究利用PUMA特异性抗体，通过Western blot和免疫组化技术验证了DNA损伤后PUMA蛋白在多种人类细胞系中的表达上调，并证实其通过激活线粒体凋亡通路诱导细胞死亡。
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2. **文献名称**：*The pro-apoptotic BH3-only protein PUMA is critical for radiation-induced gastrointestinal syndrome*
**作者**：Jeffers JR, et al.
**摘要**：通过构建PUMA基因敲除小鼠模型，结合PUMA抗体的组织染色分析，揭示了PUMA在辐射诱导的肠上皮细胞凋亡中的关键作用，为抗辐射损伤治疗提供靶点。
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3. **文献名称**：*PUMA, a novel pro-apoptotic gene, is induced by p53*
**作者**：Nakano K, Vousden KH
**摘要**：首次报道p53通过直接调控PUMA基因表达促进细胞凋亡，研究中采用PUMA抗体检测内源性蛋白表达，证实其在p53依赖性和非依赖性凋亡通路中的双重功能。
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(注：以上内容为示例，实际文献需通过PubMed、Web of Science等平台检索关键词“PUMA antibody”、“PUMA apoptosis”等获取。)
**Background of PUMA Antibody**
PUMA (p53 upregulated modulator of apoptosis) is a pro-apoptotic protein belonging to the BCL-2 family, critically involved in regulating programmed cell death. Discovered in 2001. PUMA is transcriptionally activated by p53 in response to cellular stress, such as DNA damage, hypoxia, or oncogene activation. It promotes apoptosis by binding and neutralizing anti-apoptotic BCL-2 family members, thereby activating BAX/BAK to trigger mitochondrial outer membrane permeabilization and caspase cascade activation.
PUMA antibodies are essential tools for studying apoptosis mechanisms, cancer biology, and therapeutic responses. They enable detection of PUMA expression in various experimental models, including Western blotting, immunohistochemistry (IHC), and flow cytometry. Researchers utilize these antibodies to explore PUMA's role in tumor suppression, chemosensitivity, and its dual regulation by p53-dependent and -independent pathways (e.g., via FOXO3a or E2F1).
Commercial PUMA antibodies are often validated for specificity and sensitivity across species (human, mouse, rat). Clones like EP512Y or D30C10 are widely used, with applications in both basic research and preclinical studies. PUMA's relevance in diseases, particularly cancer, underscores the importance of these antibodies in identifying biomarkers, assessing drug efficacy, and understanding resistance mechanisms linked to apoptotic evasion.
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