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Rabbit Monoclonal PUM1 Antibody

  • 中文名： PUM1抗体
  • 别    名： HsPUM; PUM L1; Pum1; PUMH 1; PUMH; PUMH1; Pumilio1; PUML1
货号: IPDX21908
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHsPUM; PUM L1; Pum1; PUMH 1; PUMH; PUMH1; Pumilio1; PUML1
Entrez GeneID9698
WB Predicted band sizeCalculated MW: 126 kDa; Observed MW: 140 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenA synthetic peptide of human Pumilio 1
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于PUM1抗体的3篇参考文献的简要列举(文献标题与作者为虚拟示例，仅供参考格式)：

1. **文献名称**: *PUM1 regulates neuronal function through RNA-binding activity and interaction with synaptic proteins*

**作者**: Smith A, et al.

**摘要**: 该研究利用PUM1特异性抗体在小鼠脑组织中发现PUM1蛋白通过与mRNA结合调控突触相关蛋白的翻译，其表达异常与神经退行性表型相关。抗体应用于免疫组化及Western blot验证了PUM1的亚细胞定位。

2. **文献名称**: *Development and validation of a high-specificity PUM1 antibody for cancer biomarker studies*

**作者**: Chen L, et al.

**摘要**: 研究团队开发了一种兔源单克隆PUM1抗体，通过ELISA和免疫荧光验证其特异性，并应用于结直肠癌组织芯片分析，发现PUM1高表达与患者预后不良显著相关。

3. **文献名称**: *PUM1-mediated RNA decay in Drosophila development: Insights from antibody-based knockdown assays*

**作者**: Tanaka K, et al.

**摘要**: 该研究利用果蝇模型，通过PUM1抗体进行免疫共沉淀(Co-IP)鉴定其结合的靶RNA分子，揭示PUM1通过促进特定mRNA降解调控胚胎发育的分子机制。

*注：以上文献为示例性质，实际引用需根据具体研究在PubMed或Google Scholar等数据库检索真实文献。*

背景信息

The PUM1 antibody is a crucial tool for studying the biological roles of PUM1 (Pumilio homolog 1), a member of the evolutionarily conserved PUF family of RNA-binding proteins. PUM1 regulates post-transcriptional gene expression by binding to specific sequences in the 3'-untranslated regions (3'-UTRs) of target mRNAs, influencing their stability, translation, or localization. It plays pivotal roles in development, neuronal function, and germline maintenance, with dysregulation linked to disorders such as cancer, neurodegenerative diseases, and developmental defects. Structurally, PUM1 contains eight tandem PUF repeats that mediate sequence-specific RNA interactions, often targeting the UGURANAU motif. The PUM1 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect PUM1 expression levels, subcellular localization, and interactions in various tissues or cell types. Its specificity is critical for distinguishing PUM1 from its paralog, PUM2. which shares overlapping functions but exhibits distinct mRNA targets. Research utilizing this antibody has advanced understanding of PUM1's involvement in mRNA decay, translational repression, and its cooperative role with other RNA-binding proteins in cellular homeostasis. Recent studies also highlight its potential as a biomarker in certain cancers and its association with PUM1-related cerebellar ataxia, a rare neurological disorder. Validation via knockout controls or peptide blocking remains essential to confirm antibody reliability.

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