首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β内啡肽(β-EP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、脑脊液（CSF）、唾液、组织匀浆样本中的β-EP含量。β-EP（β-内啡肽）是体内天然阿片肽，在痛觉调节、情绪调控等方面有重要作用。研究机制上，其能与体内阿片受体结合，产生镇痛、愉悦感等效果。对它的研究有助于深入了解疼痛、成瘾及情绪相关疾病发病机制与治疗。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于神经生物学、疼痛机制及应激反应等基础研究领域；其水平变化与神经退行性疾病、慢性疼痛综合征及精神类疾病的研究密切相关；支持科研人员对中枢神经系统、内分泌系统及相关病理模型的深入分析；样本兼容性涵盖体液与组织裂解液，特别适用于跨样本类型的对比研究，例如脑脊液与血清中β-EP的动态关联分析，或特定脑区组织匀浆的局部浓度检测；为探索β-EP在神经信号传导、应激适应机制及药物干预效应等方向提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta-Endorphin, beta-EP
缩写：β-EP
种属：Homo sapiens (Human)
样本类型：serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 91-110 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.5372.5682.5532.44320002.0101.9992.0051.89510001.6001.5321.5661.4565001.0321.1221.0770.9672500.5670.6000.5840.4741250.3910.4100.4010.29162.50.2420.2540.2480.13800.1090.1110.110 | ||||||
货期：3-5 working days
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