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Horse Beta-Endorphin,β-EP ELISA Kit

  • 中文名： 马β内啡肽(β-EP)ELISA Kit
货号: IPD-E06831Eq
Price： ￥5800产品描述： 马β内啡肽(β-EP)酶联免疫试剂盒（CSB-E06831Eq）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的β-EP含量。β-EP是重要靶点。它属内源性阿片肽，广泛分布于中枢和外周。其参与痛觉调节、情绪调控等生理过程。研究机制上，主要通过与阿片受体结合发挥作用，深入探索其机制有助于开发治疗疼痛、精神疾病等的药物。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域探究马科动物在应激状态、慢性疼痛模型、神经系统疾病或免疫调节研究中的β-EP动态变化，为揭示其生理功能及分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。 别名： beta-Endorphin, beta-EP 缩写： β-EP 种属： Equus caballus (Horse) 样本类型： serum, plasma 检测范围： 3.12 pg/mL-200 pg/mL 灵敏度： 0.78 pg/mL 反应时间： 1-5h 样本体积： 50-100ul 检测波长： 450 nm 研究领域： Cancer 测定原理： quantitative 测定方法： Sandwich 精密度： Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. 线性度： To assess the linearity of the assay, samples were spiked with high concentrations of horse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. 　 Sample Serum(n=4) 1:1 Average % 97 Range % 90-111 1:2 Average % 105 Range % 102-109 1:4 Average % 93 Range % 90-97 1:8 Average % 88 Range % 85-93 回收率： The recovery of horse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 103 99-105 EDTA plasma (n=4) 91 90-94 标准曲线： These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 200 1.899 1.922 1.911 1.788 100 1.554 1.514 1.534 1.411 50 1.123 1.078 1.101 0.978 25 0.615 0.623 0.619 0.496 12.5 0.421 0.456 0.439 0.316 6.25 0.277 0.266 0.272 0.149 3.12 0.185 0.194 0.190 0.067 0 0.126 0.120 0.123 　 数据处理： ELISA操作视频及标曲绘制软件下载 货期： 3-5 working days
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产品详情

  • 产品描述：马β内啡肽(β-EP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的β-EP含量。β-EP是重要靶点。它属内源性阿片肽，广泛分布于中枢和外周。其参与痛觉调节、情绪调控等生理过程。研究机制上，主要通过与阿片受体结合发挥作用，深入探索其机制有助于开发治疗疼痛、精神疾病等的药物。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域探究马科动物在应激状态、慢性疼痛模型、神经系统疾病或免疫调节研究中的β-EP动态变化，为揭示其生理功能及分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：beta-Endorphin, beta-EP

  • 缩写：β-EP

  • 种属：Equus caballus (Horse)

  • 样本类型：serum, plasma

  • 检测范围：3.12 pg/mL-200 pg/mL

  • 灵敏度：0.78 pg/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Cancer

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%



    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%



    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of horse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)

    1:1Average %97

    Range %90-111

    1:2Average %105

    Range %102-109

    1:4Average %93

    Range %90-97

    1:8Average %88

    Range %85-93

  • 回收率：

    The recovery of horse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample TypeAverage % RecoveryRange

    Serum (n=5)10399-105

    EDTA plasma (n=4)9190-94

















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/mlOD1OD2AverageCorrected2001.8991.9221.9111.7881001.5541.5141.5341.411501.1231.0781.1010.978250.6150.6230.6190.49612.50.4210.4560.4390.3166.250.2770.2660.2720.1493.120.1850.1940.1900.06700.1260.1200.123　







  • 货期：3-5 working days

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