首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人X-box结合蛋白1(XBP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的XBP1含量。XBP1是一种重要的靶点。它参与细胞内质网应激反应的信号通路。研究发现其在细胞分泌、代谢及免疫调节等方面发挥关键作用。异常的XBP1活性与多种疾病如癌症、代谢紊乱相关，深入研究其作用机制有助于开发相应治疗策略。试剂盒检测范围为12.5 pg/mL-800 pg/mL，可广泛应用于探索内质网应激机制、代谢综合征相关分子调控网络、神经退行性疾病模型构建以及肿瘤微环境研究等科研领域，为体外研究XBP1在细胞应激应答中的动态变化提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tax responsive element binding protein 5 ELISA Kit; Tax-responsive element-binding protein 5 ELISA Kit; TREB5 ELISA Kit; X box binding protein 1 ELISA Kit; X box binding protein 2 ELISA Kit; X-box-binding protein 1 ELISA Kit; XBP 1 ELISA Kit; XBP-1 ELISA Kit; XBP1 ELISA Kit; XBP1_HUMAN ELISA Kit; XBP2 ELISA Kit
缩写：XBP1
Uniprot No.：P17861
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human XBP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 88-100 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 93-104 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 90-104 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of human XBP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 83-95 | ||||
| EDTA plasma (n=4) | 108 | 92-112 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 8002.6092.5242.5672.439 4002.0692.0112.0401.912 2001.3741.3891.3821.254 1000.7230.7460.7350.607 500.4700.4610.4660.338 250.2680.2770.2730.145 12.50.1970.2020.2000.072 00.1300.1260.128 | ||||||
货期：3-5 working days
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