| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RD; RDP; RDBP; D6S45; NELF-E |
| Entrez GeneID | 7936 |
| WB Predicted band size | Calculated MW: 43 kDa; Observed MW: 43 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human NELFe |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇涉及NELFE抗体的参考文献概览(模拟数据)：
1. **标题**：NELFE promotes colorectal cancer progression by enhancing mRNA stability
**作者**：Zhang Y, et al.
**摘要**：研究使用NELFE抗体进行免疫沉淀，发现NELFE通过结合mRNA 3'UTR调控稳定性，促进结直肠癌细胞增殖。
2. **标题**：Immunohistochemical analysis of NELFE in hepatocellular carcinoma
**作者**：Wang L, et al.
**摘要**：通过NELFE抗体免疫组化检测肝癌组织，显示NELFE高表达与患者预后不良相关，提示其作为潜在生物标志物。
3. **标题**：NELFE interacts with HPV E7 protein in cervical carcinogenesis
**作者**：Chen X, et al.
**摘要**：利用NELFE抗体进行Co-IP实验，证实NELFE与人乳头瘤病毒E7蛋白互作，协同增强宫颈癌细胞侵袭能力。
注：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索关键词"NELFE antibody"+"specific research context"获取。建议优先选择近5年发表于《Nature Communications》《Oncogene》等期刊的研究。
NELFE (Negative Elongation Factor E), also known as RDBP, is a subunit of the Negative Elongation Factor (NELF) complex that regulates transcription by RNA polymerase II. The NELF complex, composed of NELFA, NELFB, NELFE, and NELFCD, plays a critical role in pausing RNA polymerase II during early transcription elongation, particularly in promoter-proximal regions. This pausing is a key regulatory step in gene expression, allowing fine-tuned control of transcriptional activation or repression in response to cellular signals.
NELFE antibodies are essential tools for studying the function and localization of the NELF complex in transcriptional regulation. They are widely used in techniques such as chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to investigate NELFE's interactions with chromatin, its role in gene silencing, and its involvement in developmental or disease-related processes. Dysregulation of NELF components, including NELFE, has been implicated in cancers and immune disorders, making these antibodies valuable for mechanistic studies.
Research using NELFE antibodies has helped uncover links between transcription elongation control and cellular differentiation, stress responses, and viral pathogenesis. Commercial NELFE antibodies are typically validated for specificity against conserved epitopes, though variability in post-translational modifications across cell types may require experimental optimization. Their applications span basic molecular biology and translational studies aiming to target transcription elongation machinery therapeutically.
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