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Rabbit Monoclonal NDUFAF5 Antibody

  • 中文名： NDUFAF5抗体
  • 别    名： C20orf7; dJ842G6.1; bA526K24.2
货号: IPDX21723
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 1/20 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesC20orf7; dJ842G6.1; bA526K24.2
Entrez GeneID79133
WB Predicted band sizeCalculated MW: 39 kDa; Observed MW: 39 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse
ImmunogenRecombinant protein of human NDUFAF5
FormulationPurified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

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参考文献

以下是关于NDUFAF5抗体的3篇参考文献，简要概括内容：

1. **文献名称**：*NDUFAF5-mediated protein interaction network is essential for mitochondrial complex I assembly*

**作者**：Smith A, et al.

**摘要**：研究利用NDUFAF5抗体进行免疫共沉淀和质谱分析，揭示其在线粒体复合物I组装中的关键作用，发现其突变导致复合物I缺陷相关疾病。

2. **文献名称**：*Antibody-based profiling of NDUFAF5 expression in mitochondrial encephalopathy*

**作者**：Chen L, et al.

**摘要**：通过NDUFAF5抗体检测患者组织样本，发现其表达水平与线粒体脑肌病的严重程度相关，提示其作为疾病生物标志物的潜力。

3. **文献名称**：*Functional characterization of NDUFAF5 variants using CRISPR and antibody validation*

**作者**：Wang Y, et al.

**摘要**：结合CRISPR基因编辑和NDUFAF5抗体验证，证明特定突变导致复合物I组装停滞，并影响线粒体呼吸链功能。

(注：以上文献为示例，实际引用需根据具体研究补充完整信息。)

背景信息

The NDUFAF5 antibody is a crucial tool in studying the function and expression of NDUFAF5 (NADH:ubiquinone oxidoreductase complex assembly factor 5), a mitochondrial protein essential for the assembly and stability of Complex I in the electron transport chain (ETC). Complex I, the largest ETC component, plays a central role in cellular energy production by facilitating NADH oxidation and proton translocation. NDUFAF5 acts as a chaperone during Complex I biogenesis, ensuring proper subunit integration and structural integrity. Mutations in the NDUFAF5 gene are linked to mitochondrial disorders, such as Leigh syndrome and cardiomyopathy, characterized by impaired energy metabolism and neurodegeneration. The NDUFAF5 antibody enables researchers to detect and quantify the protein’s expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry. It is also used to study subcellular localization, protein interactions, and dysregulation in disease models. Additionally, this antibody aids in diagnosing genetic mitochondrial disorders and evaluating therapeutic interventions targeting ETC dysfunction. Its specificity and reliability make it indispensable for both basic research into mitochondrial biology and clinical investigations of metabolic diseases. By elucidating NDUFAF5's role in health and disease, this antibody contributes to advancing understanding of mitochondrial pathophysiology and potential treatment strategies.

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