Mouse Vasoactive Intestinal Peptide,VIP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08356m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠血管活性肠肽(VIP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VIP含量。VIP即血管活性肠肽，是一种神经递质和旁分泌激素。它在神经调节、免疫调节、血管舒张等方面发挥作用。研究机制上，VIP可与特定受体结合，激活细胞内信号通路，影响细胞的增殖、分化及炎症反应等，在多种疾病治疗上具有潜在价值。试剂盒检测范围为7.8 pg/ml- 500 pg/ml，该产品适用于神经内分泌研究、消化系统功能探索、炎症性疾病模型构建等科研场景，例如通过检测动物模型中VIP的浓度变化，揭示其在肠道屏障调节或免疫微环境中的作用机制，为分子机理研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Vip ELISA Kit; VIP peptides [Cleaved into: Intestinal peptide PHI-42; Intestinal peptide PHI-27 ELISA Kit; Peptide histidine isoleucinamide 27); Vasoactive intestinal peptide ELISA Kit; VIP ELISA Kit; Vasoactive intestinal polypeptide)] ELISA Kit
缩写：
VIP
Uniprot No.：
P32648
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
7.8 pg/ml - 500 pg/ml
灵敏度：
1.95 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Neuroscience
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	95
	Range %	90-100
1:4	Average %	98
	Range %	92-104
1:8	Average %	85
	Range %	82-88

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
500	2.317	2.356	2.337	2.177
250	1.579	1.687	1.633	1.473
125	0.955	0.997	0.976	0.816
62.5	0.586	0.568	0.577	0.417
31.2	0.315	0.326	0.321	0.161
15.6	0.250	0.256	0.253	0.093
7.8	0.194	0.197	0.196	0.036
0	0.154	0.166	0.160