| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MAT; SAMS; MATA1; SAMS1; MAT1A |
| Entrez GeneID | 4143 |
| WB Predicted band size | Calculated MW: 44 kDa; Observed MW: 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human MAT1A |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于MAT1A抗体的3篇参考文献的简要总结：
1. **文献名称**: "Methionine adenosyltransferase 1A (MAT1A) deficiency enhances acetaminophen-induced liver injury through impaired glutathione synthesis"
**作者**: Martínez-Chantar et al.
**摘要**: 该研究利用MAT1A特异性抗体检测小鼠肝脏中MAT1A蛋白的表达，发现MAT1A缺失导致谷胱甘肽合成受损，从而加剧对乙酰氨基酚诱导的肝毒性，揭示了MAT1A在肝脏抗氧化防御中的作用。
2. **文献名称**: "MAT1A variants are associated with hypertension, stroke, and markers of DNA damage and repair in stroke patients"
**作者**: Yang et al.
**摘要**: 通过Western blot和免疫组化(使用MAT1A抗体)，研究发现MAT1A基因多态性与高血压和中风风险相关，其蛋白表达异常可能通过影响DNA修复通路参与疾病进展。
3. **文献名称**: "Methionine adenosyltransferase 1A knockout mice are predisposed to liver injury and exhibit altered expression of genes involved in lipid metabolism"
**作者**: Cai et al.
**摘要**: 利用MAT1A抗体分析基因敲除小鼠的肝组织，发现MAT1A缺失导致脂代谢相关基因表达失调，并增加酒精性肝病易感性，提示MAT1A在脂质稳态中的关键作用。
4. **文献名称**: "Epigenetic silencing of MAT1A promotes colon cancer progression by inducing ATR/CHEK1 signaling"
**作者**: Li et al.
**摘要**: 该研究通过免疫印迹(MAT1A抗体)和甲基化分析，发现结肠癌中MAT1A的表观遗传沉默激活DNA损伤信号通路，促进肿瘤增殖，为靶向MAT1A的治疗策略提供依据。
以上文献均通过MAT1A抗体探索该蛋白在疾病机制中的功能，涵盖肝脏代谢、肿瘤及DNA损伤修复等领域。
MAT1A (Methionine Adenosyltransferase 1A) is a key enzyme encoded by the MAT1A gene, primarily expressed in the liver. It catalyzes the biosynthesis of S-adenosylmethionine (SAMe), the principal methyl donor in cellular methylation reactions, which is critical for processes such as DNA/RNA modification, protein regulation, and phospholipid synthesis. MAT1A activity is tightly linked to hepatic function, including detoxification, regeneration, and metabolism. Dysregulation of MAT1A has been implicated in liver diseases (e.g., cirrhosis, hepatocellular carcinoma) and metabolic disorders due to altered SAMe levels affecting methylation homeostasis.
MAT1A antibodies are immunological tools designed to detect and quantify MAT1A protein expression in research applications. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study MAT1A's tissue-specific expression, subcellular localization, and regulatory mechanisms. They also aid in exploring MAT1A's role in disease pathogenesis, particularly in liver cancer progression, where reduced MAT1A expression correlates with poor prognosis. Researchers utilize MAT1A antibodies to investigate epigenetic changes, redox balance, and therapeutic targets related to SAMe metabolism. Specificity and validation (e.g., knockout controls) are essential to ensure accurate detection, given the homology with its isoform MAT2A.
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