| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MCM7; CDC47; MCM2; DNA replication licensing factor MCM7; CDC47 homolog; P1.1-MCM3 |
| Entrez GeneID | 4176 |
| WB Predicted band size | Calculated MW: 81 kDa; Observed MW: 81 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthetic peptide of human MCM7 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于MCM7抗体的代表性文献(均为虚构示例，实际文献需通过PubMed或学术数据库查询)：
1. **"MCM7 as a Prognostic Biomarker in Human Cancers"**
*作者：Karl Kóhler et al.*
摘要：通过免疫组化分析MCM7在多种癌症组织中的表达水平，发现其高表达与肿瘤分期、转移风险增加显著相关，提示MCM7抗体可作为预后评估工具。
2. **"Development and Validation of a Monoclonal Antibody for MCM7 Detection"**
*作者：Rosa Ana Risques et al.*
摘要：研究团队开发了一种高特异性抗MCM7单克隆抗体，验证了其在Western blot和免疫荧光中的灵敏度，并成功应用于胃癌细胞增殖活性检测。
3. **"MCM7 Overexpression Drives Genomic Instability via DNA Replication Stress"**
*作者：Eishi Shiraishi et al.*
摘要：利用MCM7抗体进行染色质免疫沉淀(ChIP)，揭示MCM7异常表达导致DNA复制压力，促进结直肠癌细胞基因组不稳定的分子机制。
4. **"Comparative Analysis of MCM7 Antibody Performance in Clinical Diagnostics"**
*作者：Li Wei et al.*
摘要：对比不同商业来源的MCM7抗体在病理切片中的染色一致性，提出标准化检测流程以提高前列腺癌诊断的准确性。
*注：以上为示例性内容，实际文献需查阅具体数据库。如需真实文献，建议使用关键词"MCM7 antibody"+"cancer"+"diagnosis"在PubMed或Web of Science检索。*
The MCM7 antibody is a key tool in studying the Mini-chromosome Maintenance (MCM) protein family, specifically targeting the MCM7 subunit. MCM proteins (MCM2-7) form a hexameric complex essential for DNA replication initiation, acting as a licensing factor to ensure genomic DNA is copied only once per cell cycle. MCM7. as a core component, plays a critical role in helicase activity, unwinding DNA during replication. Unlike many replication proteins expressed cyclically, MCM7 is present throughout the cell cycle, making it a stable marker for replication-competent cells.
In research and diagnostics, MCM7 antibodies are widely used to detect cell proliferation status, as overexpression correlates with uncontrolled cell division in cancers. Immunohistochemical staining with MCM7 antibodies helps distinguish malignant tissues (high MCM7) from normal or benign lesions (low/absent MCM7), particularly in cancers like cervical, prostate, and hepatocellular carcinoma. Its diagnostic utility extends to pre-malignant conditions, where elevated MCM7 signals early dysplastic changes. Additionally, MCM7 antibody-based assays contribute to understanding replication stress responses and chemotherapeutic resistance mechanisms. Recent studies also explore its prognostic value, linking MCM7 overexpression to poorer clinical outcomes. Commercial MCM7 antibodies are typically validated for techniques including Western blotting, immunofluorescence, and tissue microarray analysis, with careful consideration required for epitope specificity across species.
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