首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人波形蛋白(VIM)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的VIM含量。人波形蛋白（VIM）是一种III型中间丝蛋白，主要存在于非上皮细胞，特别是间充质细胞中。它通过形成网络连接细胞核、内质网和线粒体，参与细胞骨架的构建和细胞外基质的调节。VIM在细胞形态维持、细胞器锚定和细胞迁移等过程中发挥关键作用，其表达上调与上皮间质转化（EMT）等生理和病理过程相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于基础科研中探究细胞骨架动态变化、肿瘤微环境调控、纤维化疾病模型构建等方向的研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CTRCT30 ELISA Kit; Epididymis luminal protein 113 ELISA Kit; FLJ36605 ELISA Kit; HEL113 ELISA Kit; VIM ELISA Kit; VIME_HUMAN ELISA Kit; Vimentin ELISA Kit
缩写：VIM
Uniprot No.：P08670
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human VIM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 80-96 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 80-98 | |||||
| 1:800 | Average % | 100 | ||||
| Range % | 92-105 | |||||
回收率：
| The recovery of human VIM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 80-98 | ||||
| EDTA plasma (n=4) | 96 | 90-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6542.6212.6382.53210002.2242.3022.2632.1575001.5361.4871.5121.4062500.9120.8560.8840.7781250.4460.4210.4340.32862.50.2560.2410.2490.14331.250.1370.1450.1410.03500.1050.1070.106 | ||||||
货期：3-5 working days
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