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产品描述：牛运甲状腺素蛋白(TTR)酶联免疫试剂盒为竞争法 ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TTR含量。TTR（转甲状腺素蛋白）是一种血浆运载蛋白。其基因突变可致蛋白构象改变、聚集和沉积，引发ATTR淀粉样变性病，危害神经系统、心脏等。目前研究聚焦于稳定TTR结构、抑制聚集等机制，以开发针对性治疗药物。试剂盒检测范围为312.5 ng/mL-5000 ng/mL，该产品适用于牛类生理机制研究、疾病模型建立或营养代谢实验中TTR蛋白动态变化的追踪，为动物科学、兽医学及比较医学领域的科研人员提供可靠的检测工具，助力探索TTR在跨膜运输、能量代谢或组织特异性表达中的生物学功能。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：TTR ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit
缩写：TTR
Uniprot No.：O46375
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：312.5 ng/mL-5000 ng/mL
灵敏度：312.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 91-97 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 87-95 | |||||
回收率：
| The recovery of bovine TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-104 | ||||
| EDTA plasma (n=4) | 89 | 84-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average50000.1470.1530.15025000.2660.2750.27112500.5110.5120.5126250.9050.9030.904312.51.6741.7871.73102.6892.5992.644 | ||||||
货期：3-5 working days
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