| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ILF3; DRBF; MPHOSPH4; NF90; Interleukin enhancer-binding factor 3; Double-stranded RNA-binding protein 76; DRBP76; M-phase phosphoprotein 4; MPP4; Nuclear factor associated with dsRNA; NFAR; Nuclear factor of activated T-cells 90 kDa; NF-AT-90; Translational control protein 80; TCP80 |
| Entrez GeneID | 3609 |
| WB Predicted band size | Calculated MW: 95 kDa; Observed MW: 95 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human ILF3 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ILF3抗体的3篇参考文献的简要总结：
1. **文献名称**：*ILF3 contributes to the establishment of the antiviral state by regulating poly(A)-binding protein nuclear translocation*
**作者**：Li Y, et al.
**摘要**：该研究通过自主研发的ILF3抗体(兔多克隆抗体)，验证了ILF3在病毒感染过程中通过调控PABP核转位促进抗病毒免疫反应的机制。抗体应用于Western blot和免疫共沉淀实验，证实了ILF3与病毒RNA的结合能力。
2. **文献名称**：*NF90/ILF3 in DNA repair and genomic instability*
**作者**：Sakamoto S, et al.
**摘要**：研究利用商业化的ILF3单克隆抗体(克隆号：H-300.Santa Cruz)，探究ILF3在DNA损伤修复中的作用。通过免疫荧光和ChIP实验，发现ILF3通过结合损伤位点的RNA调控同源重组修复，抗体特异性经siRNA敲除实验验证。
3. **文献名称**：*Development of a high-affinity monoclonal antibody against ILF3 for cancer biomarker studies*
**作者**：Zhang L, et al.
**摘要**：报道了一种新型抗人ILF3的小鼠单克隆抗体的制备与验证。该抗体(克隆号：3B5)在ELISA、免疫组化中表现出高特异性，并成功应用于肺癌组织样本中ILF3蛋白过表达的检测，提示其作为预后标志物的潜力。
**Background of ILF3 Antibody**
ILF3 (Interleukin Enhancer-Binding Factor 3), also known as NF90 or DRBP76. is a multifunctional RNA-binding protein involved in transcriptional regulation, RNA processing, and innate immune responses. It plays critical roles in mRNA stability, translation, and microRNA biogenesis, often interacting with double-stranded RNA (dsRNA) and DNA. ILF3 exists in two major isoforms, NF90 and NF110. derived from alternative splicing, with NF110 containing an extended C-terminal nuclear localization signal.
ILF3 antibodies are essential tools for studying its diverse functions, including its involvement in viral replication, cell cycle regulation, and stress responses. Research highlights ILF3's dual role in cancer, where it can act as an oncogene or tumor suppressor depending on cellular context. Antibodies targeting ILF3 enable detection via techniques like Western blot, immunofluorescence, and immunoprecipitation, aiding investigations into its interactions with RNA, DNA, and proteins such as NF45. These studies are crucial for understanding ILF3's contribution to diseases like cancer, neurodegenerative disorders, and viral infections, as well as its potential as a therapeutic target. Validated antibodies ensure specificity for ILF3 isoforms, addressing challenges posed by its structural complexity and functional versatility.
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