首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人肌联蛋白(TTN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TTN含量。TTN（肌联蛋白）是人体内最大的蛋白质，由TTN基因编码。其突变与多种遗传性肌病和心肌病相关，如扩张型心肌病（DCM）。TTN突变通过多种机制影响心肌功能，包括改变肌联蛋白的弹性、被动张力、转录调控和翻译后修饰等，导致心肌细胞功能障碍和心脏结构异常。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，本试剂盒适用于科研领域中对心肌或骨骼肌生理功能、肌肉退行性疾病模型（如肌营养不良症）以及药物干预后肌肉修复机制的研究，可支持体外培养细胞或动物模型中TTN蛋白动态变化的分析，为探索肌肉生物学特性及病理过程提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MPRM ELISA Kit; Cardiomyopathy dilated 1G (autosomal dominant) ELISA Kit; CMD1G ELISA Kit; CMH 9 ELISA Kit; CMH9 ELISA Kit; CMPD 4 ELISA Kit; CMPD4 ELISA Kit; Connectin ELISA Kit; DKFZp451N061 ELISA Kit; EOMFC ELISA Kit; FLJ26020 ELISA Kit; FLJ26409 ELISA Kit; FLJ32040 ELISA Kit; FLJ34413 ELISA Kit; FLJ39564 ELISA Kit; FLJ43066 ELISA Kit; HMERF ELISA Kit; LGMD2J ELISA Kit; MU RMS 40.14 ELISA Kit; MYLK5 ELISA Kit; Rhabdomyosarcoma antigen ELISA Kit; Rhabdomyosarcoma antigen MU RMS 40.14 ELISA Kit; Rhabdomyosarcoma antigen MU-RMS-40.14 ELISA Kit; Titin ELISA Kit; TITIN_HUMAN ELISA Kit; TMD ELISA Kit; TTN ELISA Kit
缩写：TTN
Uniprot No.：Q8WZ42
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-104 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of human TTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-100 | ||||
| EDTA plasma (n=4) | 98 | 90-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 15002.1952.0952.1452.014 7501.5941.4941.5441.413 3750.9320.8920.9120.781 187.50.5060.4860.4960.365 940.3520.3420.3470.216 470.2290.2190.2240.093 23.50.1680.1620.1650.034 00.1310.1300.131 | ||||||
货期：3-5 working days
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