首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人热休克蛋白75 kDa，线粒体(TRAP1/HSP75)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TRAP1含量。TRAP1是重要靶点，它是热休克蛋白90家族成员，在线粒体中发挥作用。其研究机制涵盖参与线粒体代谢调控、细胞凋亡调节等。深入研究它，有望揭示疾病发生发展机制，为开发以TRAP1为靶点的治疗药物提供理论基础。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于细胞应激调控、肿瘤模型研究、线粒体代谢相关疾病机制探索等科研场景，能够为体外实验或动物模型中TRAP1/HSP75的动态变化提供稳定可靠的检测工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Heat shock protein 75 kDa ELISA Kit; Heat shock protein 75 kDa, mitochondrial ELISA Kit; HSP 75 ELISA Kit; HSP75 ELISA Kit; HSP90L ELISA Kit; mitochondrial ELISA Kit; TNF receptor associated protein 1 ELISA Kit; TNFR-associated protein 1 ELISA Kit; TRAP-1 ELISA Kit; Trap1 ELISA Kit; TRAP1_HUMAN ELISA Kit; Tumor necrosis factor type 1 receptor-associated protein ELISA Kit
缩写：TRAP1
Uniprot No.：Q12931
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human TRAP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 95-108 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 86-91 | |||||
回收率：
| The recovery of human TRAP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-95 | ||||
| EDTA plasma (n=4) | 99 | 95-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.2542.4252.3402.203 201.8341.8961.8651.728 101.3621.4221.3921.255 50.9651.0120.9890.852 2.50.6330.6290.6310.494 1.250.3820.3540.3680.231 0.6250.2660.2450.2560.119 00.1330.1400.137 | ||||||
货期：3-5 working days
×
