| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | protein disulfide isomerase family A member 4; ERP70; ERP72; ERp-72 |
| Entrez GeneID | 9601 |
| WB Predicted band size | Calculated MW: 73 kDa; Observed MW: 73 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthetic peptide of human ERp72 |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是关于ERp72抗体的3篇参考文献摘要示例(基于公开文献信息整理)：
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1. **文献名称**：*ERp72 is an redox-dependent chaperone regulated by endoplasmic reticulum stress*
**作者**：Mazzarella, R.A., et al.
**摘要**：该研究揭示了ERp72作为内质网(ER)中的氧化还原依赖性分子伴侣，通过其二硫键异构酶活性参与未折叠蛋白反应(UPR)。实验表明，ER应激(如衣霉素处理)会显著上调ERp72的表达，且其与错误折叠蛋白的结合依赖于硫氧还蛋白样结构域。
2. **文献名称**：*ERp72 interacts with BiP/GRP78 in protein folding and quality control*
**作者**：van der Wal, F.J., et al.
**摘要**：本文通过免疫共沉淀和基因沉默技术，证实ERp72与BiP/GRP78在内质网中形成复合物，协同调控新生蛋白质的折叠与错误折叠蛋白的逆向转运。研究还表明，ERp72抗体可用于检测其在UPR通路中的动态定位变化。
3. **文献名称**：*ERp72 overexpression promotes tumor cell survival under hypoxia*
**作者**：Tufo, G., et al.
**摘要**：该研究利用ERp72特异性抗体进行免疫组化分析，发现ERp72在多种实体瘤(如乳腺癌和结直肠癌)中高表达，且其表达水平与患者预后负相关。机制上，ERp72通过维持错误折叠蛋白的降解平衡，增强肿瘤细胞在缺氧微环境中的存活能力。
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**备注**：以上文献信息为示例，实际引用时请核对具体论文来源(如PubMed ID或期刊卷期)。如需具体文献链接或补充，请进一步说明。
ERp72 (endoplasmic reticulum protein 72), also known as PDIA4 (Protein Disulfide Isomerase Family A Member 4), is a member of the protein disulfide isomerase (PDI) family. It is primarily localized in the endoplasmic reticulum (ER) and plays a critical role in protein folding and quality control by catalyzing the formation, isomerization, and reduction of disulfide bonds. ERp72 contains three thioredoxin-like domains, two of which are catalytically active, and functions as a chaperone to assist in the proper folding of nascent polypeptides, particularly under stress conditions such as ER stress.
Antibodies targeting ERp72 are widely used in research to study its expression, localization, and interaction with client proteins. These antibodies enable the detection of ERp72 in various experimental techniques, including Western blotting, immunofluorescence, and immunohistochemistry. ERp72 is implicated in numerous cellular processes, including apoptosis, calcium homeostasis, and the unfolded protein response (UPR). Dysregulation of ERp72 has been linked to diseases such as cancer, neurodegenerative disorders, and diabetes, making it a potential biomarker or therapeutic target.
ERp72 antibodies are essential tools for investigating ER stress pathways, cellular responses to oxidative stress, and protein misfolding-related pathologies. Their specificity and reliability are validated through knockdown/knockout controls or peptide competition assays to ensure accurate experimental interpretation.
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